· Lorena Pantano, Ph.D. , Alexandra Bartlett, Ph.D. · Podcast · 16 min listen · Original Source
A Coffee with CompBio: Nine Samples and Zero Cells
Alex and Lorena dive into the messy reality of processing single-cell RNA-seq data.
In our first episode, Alex and Lorena dive into the messy reality of processing single-cell RNA-seq data. What started as a simple QC project turned into a week-long journey across compute environments, mysterious pipeline errors, and zero-cell outputs. Along the way, we troubleshoot issues with Cell Ranger, uncover strange sequencing artifacts, and reflect on lessons in data handling, pipeline reproducibility, and client communication.
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